Abstract
In order to study the membrane topology and the possible function of the rat liver 22 kDa integral peroxisomal membrane protein (PMP 22) at a molecular level, we have cloned PMP 22 from a lambda gt11 expression library and sequenced its cDNA. Hydropathy analysis of the deduced primary structure indicates 4 putative transmembrane segments. The accessibility to exogenous aminopeptidase of PMP 22 in intact peroxisomes suggests that the N-terminus faces the cytosol. A model of the topology of PMP 22 in the peroxisomal membrane is discussed. Homology studies revealed a striking similarity with the Mpv 17 gene product. Lack of this membrane protein causes nephrotic syndrome in mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA
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Intracellular Membranes / chemistry*
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Liver / chemistry
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Microbodies / chemistry*
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Molecular Sequence Data
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Protein Biosynthesis
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Protein Conformation
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Proteins / chemistry
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Proteins / genetics
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Rats
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Membrane Proteins
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Mpv17 protein, mouse
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Proteins
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Pxmp2 protein, mouse
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Pxmp2 protein, rat
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DNA
Associated data
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GENBANK/X68456
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GENBANK/X68457
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GENBANK/X68458
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GENBANK/X68459
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GENBANK/X68460
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GENBANK/X68461
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GENBANK/X68462
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GENBANK/X68463
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GENBANK/X70141
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GENBANK/X70223