Demonstration of acrosomal membranes using the hypo-osmotic swelling test

R Sanchez; M Concha; E Töpfer-Petersen; W B Schill

doi:10.1111/j.1439-0272.1993.tb02672.x

Demonstration of acrosomal membranes using the hypo-osmotic swelling test

Andrologia. 1993 Jan-Feb;25(1):1-2. doi: 10.1111/j.1439-0272.1993.tb02672.x.

Authors

R Sanchez¹, M Concha, E Töpfer-Petersen, W B Schill

Affiliation

¹ Department of Preclinical Sciences, University of La Frontera, Chile.

PMID: 8427416
DOI: 10.1111/j.1439-0272.1993.tb02672.x

Abstract

The state of the acrosomal membranes in human spermatozoa was studied by means of the hypo-osmotic swelling test and indirect immunofluorescence using anti-boar outer acrosomal membrane antibodies. The swelling phenomenon observed in the acrosomal region was characterized by expansion of the plasma membrane without modification of the outer acrosomal membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrosome / physiology
Acrosome / ultrastructure*
Cell Membrane / physiology*
Fluorescent Antibody Technique
Humans
Hypotonic Solutions
Male
Microscopy, Electron
Osmolar Concentration
Osmotic Pressure

Substances

Hypotonic Solutions