Abstract
A simple, fast and sensitive method to perform Southern transfer and hybridization with nonradioactive detection of genetic loci is described. With a 10-ng sample of human genomic DNA, alleles of the D2S44 locus can be detected within 7 h of completing gel electrophoresis.
MeSH terms
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Blotting, Southern / methods*
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Cell Line
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DNA / analysis*
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Deoxyribonucleases, Type II Site-Specific
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Humans
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Luminescent Measurements*
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Nucleic Acid Denaturation
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Nucleic Acid Hybridization / methods
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Ultraviolet Rays
Substances
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DNA
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Deoxyribonucleases, Type II Site-Specific
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GGCC-specific type II deoxyribonucleases