Abstract
Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP) (PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Chromosome Mapping
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Chromosomes, Fungal
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Chromosomes, Human, Pair 20*
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Cricetinae
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DNA, Single-Stranded
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Genome, Human
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Genomic Library
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Humans
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Hybrid Cells
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Mice
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Molecular Sequence Data
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Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
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Rodentia
Substances
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DNA, Single-Stranded
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Phosphoenolpyruvate Carboxykinase (GTP)
Associated data
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GENBANK/L01126
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GENBANK/L01127
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GENBANK/L01128
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GENBANK/L01129
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GENBANK/L01130
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GENBANK/L01131
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GENBANK/L12760
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GENBANK/L20583
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GENBANK/L20584
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GENBANK/X68166