Relationships between age-dependent changes in the effect of almitrine on H(+)-ATPase/ATPsynthase and the pattern of membrane fatty acid composition

Biochim Biophys Acta. 1993 Feb 8;1141(1):90-4. doi: 10.1016/0005-2728(93)90193-j.

Abstract

The effects of almitrine on ATPase/ATPsynthase previously described in beef heart mitochondria (Rigoulet et al. (1990) Biochim. Biophys. Acta 1018, 91-97) are also observed in liver mitochondria isolated from rats older than 7 weeks. In contrast, in rats younger than 5 weeks, almitrine at the same concentration has no effect on the ATPase/ATPsynthase complex. This age-dependent action of almitrine is well correlated with age-dependent modifications of two fatty acids: linoleic and docosahexaenoic acids. The possibility of a change in H+/ATP stoichiometry of the ATPase/ATPsynthase induced by almitrine seems related to more general modifications of membrane properties during growth of the rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging*
  • Almitrine / pharmacology*
  • Animals
  • Cholesterol / analysis
  • Fatty Acids / analysis*
  • Intracellular Membranes / drug effects
  • Lipid Metabolism
  • Male
  • Mitochondria, Liver / drug effects*
  • Mitochondria, Liver / enzymology
  • Mitochondria, Liver / metabolism
  • Phosphorus / analysis
  • Proton-Translocating ATPases / metabolism*
  • Rats
  • Rats, Wistar

Substances

  • Fatty Acids
  • Phosphorus
  • Cholesterol
  • Almitrine
  • Proton-Translocating ATPases