Different TBP-associated factors are required for mediating the stimulation of transcription in vitro by the acidic transactivator GAL-VP16 and the two nonacidic activation functions of the estrogen receptor

Nucleic Acids Res. 1993 Jan 11;21(1):5-12. doi: 10.1093/nar/21.1.5.

Abstract

The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonstrate that a factor(s) required for the function of the ER AFs is chromatographically separable from a factor(s) necessary for the activity of the acidic activation domain of VP16. Moreover, immunoprecipitation experiments using a monoclonal antibody directed against the TATA box binding protein (TBP) indicate, that these different factors are associated with TBP in distinct TFIID complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Precipitin Tests
  • Receptors, Estrogen / metabolism*
  • TATA Box*
  • TATA-Box Binding Protein
  • Trans-Activators / metabolism*
  • Transcription Factor TFIID
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Gal-VP16
  • Receptors, Estrogen
  • TATA-Box Binding Protein
  • Trans-Activators
  • Transcription Factor TFIID
  • Transcription Factors