Chromosome organization of Streptococcus mutans GS-5

J Gen Microbiol. 1993 Jan;139(1):67-77. doi: 10.1099/00221287-139-1-67.

Abstract

Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Chromosome Mapping*
  • Chromosomes, Bacterial*
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Electrophoresis, Gel, Pulsed-Field
  • Genome, Bacterial
  • Mutagenesis, Insertional
  • Nucleic Acid Hybridization
  • Restriction Mapping
  • Streptococcus mutans / genetics*

Substances

  • DNA, Bacterial
  • CCCGGG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific
  • GCGGCCGC-specific type II deoxyribonucleases
  • GGGCCC-specific type II deoxyribonucleases