Abstract
A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine T-cell antigen receptor beta chains were measured for all possible V beta-J beta combinations. This analysis shows that beta chains are distributed into at least 2000 groups, a value that provides a lower limit to their complexity. The CDR3 sizes appear to be dependent on the J beta and especially the V beta segment used and correlates with amino acid sequence motifs in the corresponding CDR1 region. This feature of T-cell receptors is discussed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cloning, Molecular
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Codon / genetics
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DNA / genetics
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DNA / isolation & purification
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Genetic Variation*
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Haplotypes
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Major Histocompatibility Complex
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Molecular Sequence Data
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Molecular Weight
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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Receptors, Antigen, T-Cell, alpha-beta / genetics*
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Receptors, Antigen, T-Cell, alpha-beta / isolation & purification
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Recombination, Genetic*
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Sequence Homology, Amino Acid
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T-Lymphocytes / immunology*
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Thymus Gland / immunology
Substances
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Codon
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Oligodeoxyribonucleotides
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Receptors, Antigen, T-Cell, alpha-beta
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DNA