Abstract
An adoptive transfer immunization/fusion protocol in mice has been successfully used to raise a series of monoclonal antibodies directed against the Human Interleukin for DA-1a (HILDA)/Leukemia Inhibitory Factor (LIF) cytokine. These antibodies which were raised using recombinant HILDA/LIF purified from conditioned medium of transfected Chinese Hamster Ovary (CHO) cells also react with natural HILDA/LIF from the HSB2 T lymphoma cell line and unglycosylated HILDA/LIF produced in E. coli. They define four separate epitopes, one of which is involved in receptor binding and induction of biological activity. A sensitive sandwich immunoradiometric assay which is linear up to 5 ng/ml HILDA/LIF and can detect as low as 25 pg/ml of the cytokine has been developed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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CHO Cells
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Cell Division / physiology
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Cricetinae
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Growth Inhibitors / analysis
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Growth Inhibitors / immunology*
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Growth Inhibitors / metabolism
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Immunoglobulin Isotypes
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Immunoradiometric Assay
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Interleukin-6*
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lymphokines / analysis
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Lymphokines / immunology*
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Lymphokines / metabolism
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Precipitin Tests
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Receptors, Cytokine*
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Receptors, Immunologic / metabolism
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Receptors, OSM-LIF
Substances
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Antibodies, Monoclonal
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Growth Inhibitors
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Immunoglobulin Isotypes
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Interleukin-6
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LIF protein, human
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LIFR protein, human
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lymphokines
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Receptors, Cytokine
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Receptors, Immunologic
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Receptors, OSM-LIF