Identification of 2-amino-2,6-dideoxy-D-glucose (D-quinovosamine), isolated from the cell walls of the alkaliphilic Bacillus sp. Y-25, by 500-MHz 1H NMR spectroscopy

M Ito; R Aono; K Horikoshi

doi:10.1016/0008-6215(93)80032-a

Identification of 2-amino-2,6-dideoxy-D-glucose (D-quinovosamine), isolated from the cell walls of the alkaliphilic Bacillus sp. Y-25, by 500-MHz 1H NMR spectroscopy

Carbohydr Res. 1993 Apr 7:242:173-80. doi: 10.1016/0008-6215(93)80032-a.

Authors

M Ito¹, R Aono, K Horikoshi

Affiliation

¹ Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

PMID: 8495438
DOI: 10.1016/0008-6215(93)80032-a

Abstract

Cell walls of alkaliphilic Bacillus strain Y-25 are composed of gamma-peptidoglycan and two acidic polymers. An amino sugar, which was a min component of one acidic polymer, did not correspond to any of the commercially available hexosamines. The amino sugar was isolated from the hydrolysate of the acidic polymer, purified, and identified as D-quinovosamine (2-amino-2,6- dideoxy-D-glucose) by 500-MHz NMR spectroscopic analysis and polarimetry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / chemistry*
Carbohydrates / analysis
Cell Wall / chemistry
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Glucosamine / analogs & derivatives*
Glucosamine / analysis
Glucosamine / isolation & purification
Hydrogen
Magnetic Resonance Spectroscopy / methods
Peptidoglycan / chemistry*
Peptidoglycan / isolation & purification

Substances

Carbohydrates
Peptidoglycan
Hydrogen
Glucosamine
6-desoxy-D-glucosamine