Participation of tyrosine kinase in capping, internalization, and antigen presentation through membrane immunoglobulin in BAL17 B lymphoma cells

K Shimo; Y Gyotoku; Y Arimitsu; T Kakiuchi; J Mizuguchi

doi:10.1016/0014-5793(93)81473-d

Participation of tyrosine kinase in capping, internalization, and antigen presentation through membrane immunoglobulin in BAL17 B lymphoma cells

FEBS Lett. 1993 May 24;323(1-2):171-4. doi: 10.1016/0014-5793(93)81473-d.

Authors

K Shimo¹, Y Gyotoku, Y Arimitsu, T Kakiuchi, J Mizuguchi

Affiliation

¹ Department of Immunology and Serology, Tokyo Medical College, Japan.

PMID: 8495734
DOI: 10.1016/0014-5793(93)81473-d

Abstract

BAL17 cells pulsed with goat anti-IgM or anti-IgD as antigens stimulated a goat IgG specific T cell clone in terms of inositol phosphate production. The antigen-presenting capacity of BAL17 cells was inhibited by pretreatment with the tyrosine kinase inhibitors herbimycin A or genistein. Furthermore, ligand-induced capping and endocytosis of membrane immunoglobulin, monitored at the single cell level, was also blocked by herbimycin A. These results indicate that tyrosine phosphorylation plays an important role in receptor-mediated antigen presentation by B cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibiotics, Antineoplastic / pharmacology
Antigen-Presenting Cells / enzymology
Antigen-Presenting Cells / metabolism*
Benzoquinones
Endocytosis
Enzyme Activation
Immunologic Capping*
Lactams, Macrocyclic
Lymphoma, B-Cell
Protein-Tyrosine Kinases / metabolism*
Quinones / pharmacology
Receptors, Antigen, B-Cell / metabolism*
Rifabutin / analogs & derivatives
Tumor Cells, Cultured

Substances

Antibiotics, Antineoplastic
Benzoquinones
Lactams, Macrocyclic
Quinones
Receptors, Antigen, B-Cell
Rifabutin
herbimycin
Protein-Tyrosine Kinases