Abstract
Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
ATPases Associated with Diverse Cellular Activities
-
Amino Acid Sequence
-
Chromatography, High Pressure Liquid
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / isolation & purification
-
DNA-Binding Proteins / metabolism*
-
Electrophoresis, Polyacrylamide Gel
-
Gene Expression Regulation, Viral
-
Gene Products, tat / metabolism*
-
HIV / genetics*
-
HIV / metabolism*
-
Humans
-
Macromolecular Substances
-
Molecular Sequence Data
-
Peptide Fragments / isolation & purification
-
Peptide Hydrolases / genetics
-
Peptide Hydrolases / isolation & purification
-
Peptide Hydrolases / metabolism*
-
Proteasome Endopeptidase Complex*
-
Sequence Homology, Amino Acid
-
Transcriptional Activation*
-
tat Gene Products, Human Immunodeficiency Virus
Substances
-
DNA-Binding Proteins
-
Gene Products, tat
-
Macromolecular Substances
-
Peptide Fragments
-
tat Gene Products, Human Immunodeficiency Virus
-
PSMC2 protein, human
-
Peptide Hydrolases
-
Proteasome Endopeptidase Complex
-
ATP dependent 26S protease
-
ATPases Associated with Diverse Cellular Activities