Peptide sequencing identifies MSS1, a modulator of HIV Tat-mediated transactivation, as subunit 7 of the 26 S protease

FEBS Lett. 1993 Jun 1;323(3):276-8. doi: 10.1016/0014-5793(93)81356-5.

Abstract

Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Viral
  • Gene Products, tat / metabolism*
  • HIV / genetics*
  • HIV / metabolism*
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • Peptide Fragments / isolation & purification
  • Peptide Hydrolases / genetics
  • Peptide Hydrolases / isolation & purification
  • Peptide Hydrolases / metabolism*
  • Proteasome Endopeptidase Complex*
  • Sequence Homology, Amino Acid
  • Transcriptional Activation*
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • DNA-Binding Proteins
  • Gene Products, tat
  • Macromolecular Substances
  • Peptide Fragments
  • tat Gene Products, Human Immunodeficiency Virus
  • PSMC2 protein, human
  • Peptide Hydrolases
  • Proteasome Endopeptidase Complex
  • ATP dependent 26S protease
  • ATPases Associated with Diverse Cellular Activities