Abstract
We describe a method for the typing and quantitation of Klebsiella pneumoniae serotype O1 lipopolysaccharide (LPS) based on inhibition in an enzyme-linked immunosorbent assay of a reaction of known O1 LPS antigen and anti-O1 antibody by unknown LPS extracts. Serotype O1 was found in 32% of the 124 K. pneumoniae clinical isolates tested, showing that this serotype is frequent among the eight O serotypes which have been described previously.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques / statistics & numerical data
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Evaluation Studies as Topic
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Humans
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Klebsiella pneumoniae / chemistry
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Klebsiella pneumoniae / classification*
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Klebsiella pneumoniae / isolation & purification
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Lipopolysaccharides / analysis
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Lipopolysaccharides / classification*
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Sensitivity and Specificity
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Serotyping