Abstract
A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and that of phospholipase A2 activity. Prolactin secretion from GH3 cells is also blocked by a membrane-permeable phospholipase A2 inhibitor, suggesting the physiological relevance to inhibition of protein transport in vitro by phospholipase A2 inhibitors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / metabolism
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Animals
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Biological Transport / drug effects
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Brefeldin A
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Cell-Free System
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Cells, Cultured
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Cyclopentanes / pharmacology
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Fatty Acids / pharmacology
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism*
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Masoprocol / pharmacology
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Membrane Glycoproteins*
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Phospholipases A / analysis*
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Phospholipases A / antagonists & inhibitors
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Phospholipases A2
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Prolactin / metabolism
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Viral Envelope Proteins / metabolism*
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ortho-Aminobenzoates / pharmacology
Substances
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Cyclopentanes
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Fatty Acids
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G protein, vesicular stomatitis virus
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Membrane Glycoproteins
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Viral Envelope Proteins
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ortho-Aminobenzoates
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Brefeldin A
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Masoprocol
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Prolactin
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Phospholipases A
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Phospholipases A2
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Acetylglucosamine