Abstract
In view of the growing evidence that virus-specific cytotoxic T lymphocytes (CTL) play an important role in containing the early spread of human immunodeficiency virus type 1 (HIV-1) in infected individuals, novel vaccine strategies capable of eliciting HIV-1-specific CTL are being pursued in attempts to create an effective AIDS vaccine. We have used the simian immunodeficiency virus of macaques (SIVmac)/rhesus monkey model to explore the induction of AIDS virus-specific CTL responses by DNA vaccination. We found that the inoculation of rhesus monkeys with plasmid DNA encoding SIVmac Env and Gag elicited a persisting SIVmac-specific memory CTL response. These CTL were CD8+ and major histocompatibility complex class I restricted. These studies provide evidence for the potential utility of DNA inoculation as an approach to an HIV-1 vaccine.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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DNA, Viral / administration & dosage
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DNA, Viral / immunology*
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Gene Products, env / genetics
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Gene Products, env / immunology
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Histocompatibility Antigens Class I / immunology
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Macaca mulatta
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Plasmids
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Retroviridae Proteins / genetics
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Retroviridae Proteins / immunology
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Simian Immunodeficiency Virus / genetics
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Simian Immunodeficiency Virus / immunology*
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T-Lymphocytes, Cytotoxic / immunology*
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Tumor Cells, Cultured
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
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Viral Vaccines / administration & dosage
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Viral Vaccines / immunology*
Substances
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DNA, Viral
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Gene Products, env
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Histocompatibility Antigens Class I
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Retroviridae Proteins
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SIV envelope protein gp110
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Vaccines
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simian immunodeficiency virus gp130