Detection of MspI RFLP in human THY1 gene by the polymerase chain reaction

Jpn J Hum Genet. 1995 Sep;40(3):279-80. doi: 10.1007/BF01876187.

Abstract

THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 11q23 has been implicated in susceptibility to type 1 diabetes (Wong et al., 1991). Two primers derived from the sequences flanking the polymorphic MspI site in intron 2 of the human THY1 gene (Gatti et al., 1988) were selected for RCP to amplify a 566 bp fragment that spans the MspI polymorphism. Polymorphism was detected by MspI digestion of the PCR product.

MeSH terms

  • Base Sequence
  • Deoxyribonuclease HpaII
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length*
  • Thy-1 Antigens / genetics*

Substances

  • Thy-1 Antigens
  • Deoxyribonuclease HpaII