Recombinant enzymes for islet isolation: purification of a collagenase from Clostridium histolyticum and cloning/expression of the gene

MeSH terms

• Amino Acid Sequence
• Animals
• Cell Separation / methods*
• Chromosome Mapping
• Cloning, Molecular
• Clostridium / enzymology*
• Collagenases / classification
• Collagenases / genetics*
• Collagenases / isolation & purification*
• Escherichia coli / genetics
• Gene Expression
• Genes, Bacterial*
• Islets of Langerhans / cytology*
• Molecular Sequence Data
• Oligopeptides / chemistry
• Plasmids / genetics
• Recombinant Proteins / genetics
• Recombinant Proteins / isolation & purification
• Substrate Specificity
• Swine