Abstract
T cells play an important role in the pathogenesis of diabetes in the nonobese diabetic (NOD) mouse. CD8 cytotoxic T cell lines and clones were generated from the lymphocytic infiltrate in the islets of Langerhans of young (7-wk-old). NOD mice by growing them on (NOD x B6-RIP-B7-1)F1 islets. These cells proliferate specifically to NOD islets and kill NOD islets in vitro. The cells are restricted by H-2Kd, and all bear T cell antigen receptor encoded by V beta 6. When these CD8 T cell lines and clones are adoptively transferred to irradiated female NOD, young NOD-SCID, and CB17-SCID mice, diabetes occurs very rapidly, within 10 d of transfer and without CD4 T cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B7-1 Antigen / genetics
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B7-1 Antigen / immunology*
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Base Sequence
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CD4-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / immunology*
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Clone Cells
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Cytokines / biosynthesis
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Diabetes Mellitus, Type 2 / immunology*
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Female
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Immunohistochemistry
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Immunotherapy, Adoptive
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Insulin / genetics
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Islets of Langerhans / immunology*
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Lymphocyte Activation
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Membrane Glycoproteins / biosynthesis
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred NOD
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Mice, SCID
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Molecular Sequence Data
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Pancreas / anatomy & histology
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Perforin
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Pore Forming Cytotoxic Proteins
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Promoter Regions, Genetic / genetics
Substances
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B7-1 Antigen
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Cytokines
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Insulin
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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Perforin