Cloning and characterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor

Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):497-501. doi: 10.1073/pnas.93.1.497.

Abstract

Interleukins 4 (IL-4) and 13 (IL-13) have been found previously to share receptor components on some cells, as revealed by receptor cross-competition studies. In the present study, the cloning is described of murine NR4, a previously unrecognized receptor identified on the basis of sequence similarity with members of the hemopoietin receptor family. mRNA encoding NR4 was found in a wide range of murine cells and tissues. By using transient expression in COS-7 cells, NR4 was found to encode the IL-13 receptor alpha chain, a low-affinity receptor capable of binding IL-13 but not IL-4 or interleukins 2, -7, -9, or -15. Stable expression of the IL-13 receptor alpha chain (NR4) in CTLL-2 cells resulted in the generation of high-affinity IL-13 receptors capable of transducing a proliferative signal in response to IL-13 and, moreover, led to competitive cross-reactivity in the binding of IL-4 and IL-13. These results suggest that the IL-13 receptor alpha chain (NR4) is the primary binding subunit of the IL-13 receptor and may also be a component of IL-4 receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / chemistry*
  • Antigens, CD / genetics
  • Base Sequence
  • Cell Division
  • Cells, Cultured
  • DNA, Complementary / genetics
  • Genes
  • Interleukin-13 / metabolism
  • Interleukin-13 Receptor alpha1 Subunit
  • Interleukin-4 / metabolism
  • Mice
  • Molecular Sequence Data
  • Receptors, Interleukin / chemistry*
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4
  • Recombinant Proteins / metabolism
  • Transfection

Substances

  • Antigens, CD
  • DNA, Complementary
  • Il13ra1 protein, mouse
  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • Receptors, Interleukin
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4
  • Recombinant Proteins
  • Interleukin-4

Associated data

  • GENBANK/S80963