Abstract
It is known that down-regulation of cell surface platelet-derived growth factor (PDGF) receptors accompanies transformation by SV40. In this work human embryonic lung fibroblasts were used as a model system to study the effects of SV 40 on PDGF receptor expression. It is shown that transformation by SV 40 early region leads to a total loss of PDGF alpha-receptor and partial loss of beta-receptor mRNA. Microinjection experiments revealed that receptor down-regulation was a primary effect, and not only secondary to transformation and clonal selection. Total loss of PDGF alpha-receptor expression requires both large T and small t, and down-regulation of the PDGF alpha-receptor occurs independently of p53 and Rb binding to large T.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Polyomavirus Transforming / physiology*
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Binding Sites / physiology
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Down-Regulation / physiology
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Embryo, Mammalian / cytology
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Fibroblasts / cytology
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Fibroblasts / ultrastructure
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Gene Expression Regulation, Viral / physiology
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Humans
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Lung / cytology
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Mutation / physiology
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Polyomavirus / immunology*
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RNA, Messenger / metabolism
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Receptors, Platelet-Derived Growth Factor / antagonists & inhibitors
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Receptors, Platelet-Derived Growth Factor / genetics*
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Retinoblastoma Protein / metabolism
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Time Factors
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Transformation, Genetic / physiology
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Tumor Suppressor Protein p53 / metabolism
Substances
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Antigens, Polyomavirus Transforming
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RNA, Messenger
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Retinoblastoma Protein
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Tumor Suppressor Protein p53
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Receptors, Platelet-Derived Growth Factor