FISH and PRINS, a strategy for rapid chromosome screening: application to the assessment of aneuploidy in human sperm

Cytogenet Cell Genet. 1996;72(1):34-6. doi: 10.1159/000134155.

Abstract

The co-utilization of FISH and PRINS techniques for in situ chromosome screening was tested on human sperm nuclei. We used a centromeric repeat probe specific for chromosome 4 for FISH. PRINS reactions were performed with alpha-satellite primers specific for either chromosomes 9 or chromosome 18. Double labeling was obtained and estimates of disomy rates were carried out for the three chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aneuploidy*
  • Base Sequence
  • Chromosome Aberrations / diagnosis
  • Chromosome Disorders
  • Chromosome Mapping
  • DNA Primers / chemistry
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Male
  • Microscopy, Fluorescence / methods*
  • Molecular Sequence Data
  • Spermatozoa / ultrastructure*

Substances

  • DNA Primers