Objective: To describe a method for the training of personnel and the implementation of intracytoplasmic sperm injection (ICSI) into an IVF program. The results of the first 75 cycles are reviewed.
Design: Retrospective review of the first 75 consecutive ICSI procedures.
Setting: Private, community-based IVF program.
Main outcome measures: The fertilization rate, damage rate, ongoing pregnancy rate (PR), and implantation rate were measured.
Results: Nine percent of the injected oocytes were damaged. The fertilization rate was 60%, and the cleavage rate was 98%. Fifty-nine percent of the cycles resulted in an ongoing pregnancy, and the implantation rate per embryo was 26%.
Conclusions: A high initial PR can be obtained with ICSI using a systematic training regimen.