Abstract
By employing immunological methods, it has been demonstrated that myosin, myosin light chain (MLC) and myosin light chain kinase (MLCK) proteins in outer hair cells (OHC) are immunologically different from isoforms in platelets, smooth muscle and heart muscle, and are probably more related to isoforms found in red blood cells (RBC). Moreover, proteins related to band 3 protein (b3p) and protein 4.1 (p 4.1), ankyrin as well as fodrin and spectrin, but not glycophorin, have been identified in isolated OHCs. Both OHCs and RBC differ from other motile non-muscle cells in their lack of smooth muscle isoforms of actin, their common high levels of spectrin-, ankyrin- and band 3-like proteins, as well as the expression of the 80 kDa protein 4.1 isoform. The data support the notion that motility of OHC may be based upon regulation of the b3p/p 4.1/ankyrin complex, and thus may be reminiscent to the active shape changes in RBC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / biosynthesis
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Actins / immunology
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Animals
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Ankyrins / biosynthesis
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Ankyrins / immunology
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Blood Platelets / enzymology
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Blood Platelets / metabolism
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Carrier Proteins / biosynthesis
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Carrier Proteins / immunology
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Erythrocytes / enzymology
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Erythrocytes / metabolism
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Female
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Guinea Pigs
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Hair Cells, Auditory, Outer / cytology*
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Hair Cells, Auditory, Outer / enzymology
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Hair Cells, Auditory, Outer / metabolism
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Immunoblotting
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Isoenzymes
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Male
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Microfilament Proteins / biosynthesis
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Microfilament Proteins / immunology
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Muscle, Smooth, Vascular / enzymology
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Muscle, Smooth, Vascular / metabolism
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Myocardium / enzymology
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Myocardium / metabolism
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Myosin Light Chains / biosynthesis*
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Myosin Light Chains / immunology
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Myosin Light Chains / metabolism
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Myosin-Light-Chain Kinase / biosynthesis*
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Myosin-Light-Chain Kinase / immunology
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Myosin-Light-Chain Kinase / metabolism
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Myosins / biosynthesis*
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Myosins / immunology
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Myosins / metabolism
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / immunology
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Organ of Corti / cytology
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Organ of Corti / metabolism
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Spectrin / biosynthesis
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Spectrin / immunology
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Stereoisomerism
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Vestibule, Labyrinth / enzymology
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Vestibule, Labyrinth / metabolism
Substances
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Actins
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Ankyrins
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Carrier Proteins
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Isoenzymes
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Microfilament Proteins
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Myosin Light Chains
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Nerve Tissue Proteins
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fodrin
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Spectrin
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Myosin-Light-Chain Kinase
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Myosins