T-vector cloning and high performance PCR with SuperTth from Thermus thermophilus

J F Leal; J López-Barea; G Dorado

doi:10.1016/1050-3862(95)00118-2

T-vector cloning and high performance PCR with SuperTth from Thermus thermophilus

Genet Anal. 1995 Oct;12(2):119-21. doi: 10.1016/1050-3862(95)00118-2.

Authors

J F Leal¹, J López-Barea, G Dorado

Affiliation

¹ Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Córdoba, Spain.

PMID: 8574896
DOI: 10.1016/1050-3862(95)00118-2

Abstract

The SuperTth DNA polymerase from Thermus thermophilus exhibits template-independent terminal transferase (extendase) activity. This enzyme is proposed as a cheap alternative for both high performance PCR as well as quick T-vector cloning of amplicons, including reverse transcription and cDNA cloning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cloning, Molecular / methods*
DNA, Recombinant
DNA-Directed DNA Polymerase / genetics
DNA-Directed DNA Polymerase / metabolism*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Thermus thermophilus / enzymology*

Substances

DNA, Recombinant
DNA polymerase, Thermus thermophilus
DNA-Directed DNA Polymerase