Abstract
The fct cbsCEBA operon from the Erwinia chrysanthemi 3937 chrysobactin-dependent iron assimilation system codes for transport and biosynthetic functions. The sequence of the fct outer membrane receptor gene was determined. The fct promoter region displays a strong resemblance to the Escherichia coli bidirectional intercistronic region controlling the expression of the fepA-entD and fes-entF operons. An apparent Fur-binding site was shown to confer iron regulation on an fct::lac fusion expressed on a low-copy-number plasmid in a Fur-proficient E. coli strain. The fct gene consists of an open reading frame encoding a 735-amino-acid polypeptide with a signal sequence of 38 residues. The Fct protein has 36% sequence homology with the E. coli ferrichrome receptor FhuA and the Yersinia enterocolitica ferrioxamine receptor FoxA. On the basis of secondary-structure predictions and these homologies, we propose a two-dimensional folding model for Fct.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Carboxylic Ester Hydrolases / genetics
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Carrier Proteins / genetics
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Dickeya chrysanthemi / genetics*
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Dipeptides / metabolism
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Escherichia coli Proteins*
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Ferric Compounds / metabolism
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Iron / metabolism
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Operon
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Promoter Regions, Genetic
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Protein Structure, Secondary
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Receptors, Cell Surface / genetics*
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Receptors, Virus / genetics
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Repressor Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Siderophores / metabolism
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Transcription, Genetic
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Carrier Proteins
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Dipeptides
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Escherichia coli Proteins
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Ferric Compounds
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FhuA protein, E coli
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FoxA protein, bacteria
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FoxB protein, E coli
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Membrane Proteins
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Receptors, Cell Surface
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Receptors, Virus
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Repressor Proteins
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Siderophores
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enterobactin receptor
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ferric uptake regulating proteins, bacterial
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ferrichysobactin receptor, Pectobacterium chrysanthemi
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tonB protein, Bacteria
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tonB protein, E coli
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chrysobactin
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Iron
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Carboxylic Ester Hydrolases