Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems

Methods Enzymol. 1995:257:3-9. doi: 10.1016/s0076-6879(95)57003-9.

Authors

C Nuoffer¹, F Peter, W E Balch

Affiliation

¹ Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

PMID: 8583932
DOI: 10.1016/s0076-6879(95)57003-9

No abstract available

MeSH terms

Animals
Cell Line
Cell Membrane / metabolism
Chromatography, Affinity / methods
Chromatography, Gel / methods
Chromatography, Ion Exchange / methods
Cloning, Molecular / methods
Escherichia coli
GTP Phosphohydrolases / biosynthesis
GTP Phosphohydrolases / isolation & purification*
GTP-Binding Proteins / biosynthesis
GTP-Binding Proteins / isolation & purification*
Gene Expression*
Genetic Vectors
Histidine*
Recombinant Proteins / biosynthesis
Recombinant Proteins / isolation & purification
Spodoptera
Transfection / methods
rab1 GTP-Binding Proteins

Substances

Recombinant Proteins
Histidine
GTP Phosphohydrolases
GTP-Binding Proteins
RAB1B protein, rat
rab1 GTP-Binding Proteins