Expression and purification of recombinant His6-tagged guanine nucleotide dissociation inhibitor and formation of its Rab1 complex

Methods Enzymol. 1995:257:80-3. doi: 10.1016/s0076-6879(95)57012-8.

Authors

F Peter¹, C Nuoffer, I Schalk, W E Balch

Affiliation

¹ Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

PMID: 8583941
DOI: 10.1016/s0076-6879(95)57012-8

No abstract available

MeSH terms

Animals
Cattle
Chromatography, Affinity / methods
Chromatography, Gel / methods
Cloning, Molecular / methods
Electrophoresis, Polyacrylamide Gel / methods
Escherichia coli
GTP Phosphohydrolases / biosynthesis
GTP Phosphohydrolases / isolation & purification
GTP Phosphohydrolases / metabolism*
GTP-Binding Proteins / biosynthesis*
GTP-Binding Proteins / isolation & purification*
GTP-Binding Proteins / metabolism
Guanine Nucleotide Dissociation Inhibitors*
Histidine
Indicators and Reagents
Molecular Weight
Recombinant Fusion Proteins / biosynthesis
Recombinant Proteins / biosynthesis*
Recombinant Proteins / isolation & purification
Sequence Tagged Sites

Substances

GDP dissociation inhibitor 1
Guanine Nucleotide Dissociation Inhibitors
Indicators and Reagents
Recombinant Fusion Proteins
Recombinant Proteins
Histidine
GTP Phosphohydrolases
GTP-Binding Proteins