Abstract
Heterotrimeric guanine nucleotide binding protein (G protein)-linked receptors of the chemoattractant subfamily can trigger adhesion through leukocyte integrins, and in this role they are thought to regulate immune cell-cell interactions and trafficking. In lymphoid cells transfected with formyl peptide or interleukin-8 receptors, agonist stimulation activated nucleotide exchange on the small guanosine triphosphate-binding protein RhoA in seconds. Inactivation of Rho by C3 transferase exoenzyme blocked agonist-induced lymphocyte alpha4beta1 adhesion to vascular cell adhesion molecule-1 and neutrophil beta2 integrin adhesion to fibrinogen. These findings suggest that Rho participates in signaling from chemoattractant receptors to trigger rapid adhesion in leukocytes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD / genetics
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B-Lymphocytes / physiology*
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Cell Adhesion*
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Cells, Cultured
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Chemotactic Factors / pharmacology*
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GTP-Binding Proteins / metabolism
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GTP-Binding Proteins / physiology*
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
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Guanosine Diphosphate / metabolism
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Integrin alpha4beta1
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Integrins / physiology*
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Interleukin-8 / pharmacology
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Mice
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Molecular Sequence Data
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Receptors, Formyl Peptide
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Receptors, Immunologic / genetics
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Receptors, Interleukin / genetics
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Receptors, Interleukin-8A
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Receptors, Lymphocyte Homing / physiology*
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Receptors, Peptide / genetics
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Signal Transduction
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Tetradecanoylphorbol Acetate / pharmacology
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Transfection
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Vascular Cell Adhesion Molecule-1 / physiology*
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rhoA GTP-Binding Protein
Substances
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Antigens, CD
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Chemotactic Factors
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Integrin alpha4beta1
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Integrins
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Interleukin-8
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Receptors, Formyl Peptide
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Receptors, Immunologic
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Receptors, Interleukin
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Receptors, Interleukin-8A
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Receptors, Lymphocyte Homing
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Receptors, Peptide
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Vascular Cell Adhesion Molecule-1
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Guanosine Diphosphate
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Guanosine 5'-O-(3-Thiotriphosphate)
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N-Formylmethionine Leucyl-Phenylalanine
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GTP-Binding Proteins
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rhoA GTP-Binding Protein
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Tetradecanoylphorbol Acetate