Purification, characterization, crystallization, and preliminary X-ray results from Paracoccus denitrificans porin

Proteins. 1995 Oct;23(2):282-4. doi: 10.1002/prot.340230217.

Abstract

The porin from Paracoccus denitrificans ATCC 13543 was purified and crystallized. Two crystal forms were obtained from porin solutions with beta-d-octylglucopyranoside as detergent. Crystals of form I belong to the monoclinic spacegroup C2 with unit cell dimensions a = 112.2 A, b = 193.8 A, c = 100.5 A and beta = 129.2 degrees. There is 1 trimer per asymmetric unit. Crystals of form II are triclinic with a = 89.7 A, b = 98.8 A, c = 112.5 A, alpha = 112.5 degrees, beta = 101.8 degrees, gamma = 106.7 degrees (2 trimers per asymmetric unit). Both crystal forms diffract to 3 A.

Publication types

  • Comparative Study

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Detergents
  • Glucosides
  • Paracoccus denitrificans / chemistry*
  • Porins / chemistry*
  • Porins / isolation & purification
  • Protein Conformation

Substances

  • Detergents
  • Glucosides
  • Porins
  • octyl-beta-D-glucoside