Abstract
We have used differential phospholipid subclass labelings performed with [3H]lyso PAF and [3H] myristic acid into vascular smooth muscle cells (vSMC) to characterize the subclasses of phospholipid substrates upon different stimulation times with platelet-derived growth factor (PDGF-BB). In cells labeled with [3H]lyso PAF, PDGF-BB induced a sustained hydrolysis of alkyl-PE. In contrast, in [3H]myristic acid-labeled cells, PDGF-BB promoted a rapid and transitory hydrolysis of diacyl-PC. This hydrolysis was concomitant with an synthesis of diglyceride (DG) and phosphatidic acid (PA). Thus, both diacyl-PC and alkyl-PE appear to be major targets in PDGF-BB stimulation of SMC. These results suggest that agonists could induce the hydrolysis of precise phospholipid subclasses leading to a new specificity into the signal transduction cascade.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta, Thoracic / metabolism*
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Becaplermin
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Cells, Cultured
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Hydrolysis
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Kinetics
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Myristic Acid
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Myristic Acids / metabolism
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Phospholipids / classification
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Phospholipids / isolation & purification
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Phospholipids / metabolism*
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Platelet Activating Factor / analogs & derivatives
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Platelet Activating Factor / metabolism
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Platelet-Derived Growth Factor / pharmacology*
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Proto-Oncogene Proteins c-sis
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Radioisotope Dilution Technique
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Recombinant Proteins / pharmacology
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Swine
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Time Factors
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Tritium
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Tunica Media / drug effects
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Tunica Media / metabolism*
Substances
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Myristic Acids
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O-deacetyl platelet activating factor
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Phospholipids
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Platelet Activating Factor
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-sis
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Recombinant Proteins
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Myristic Acid
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Tritium
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Becaplermin