Abstract
Neutrophil chemotactic factors (chemokines) have been purified from conditioned medium of lipopolysaccharide (LPS)-stimulated rat macrophages in culture. The LPS-stimulated macrophages produced one acidic chemokine, rat macrophage inflammatory protein (MIP)-1alpha, and four basic chemokines, cytokine-induced neutrophil chemoattractant (CINC)-1. CINC-2alpha, CINC-2beta and CINC-3/rat MIP-2. CINC-2alpha, a novel chemokine recently isolated from conditioned medium of rat granulation-tissue culture, was the major chemoattractant among these four basic chemokines. The results suggest that CINC-2alpha is produced by activated macrophages in vivo and plays an important role in the infiltration of neutrophils into inflammatory sites in rats
MeSH terms
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Amino Acid Sequence
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Animals
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Cells, Cultured
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Chemokine CXCL1
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Chemokine CXCL2
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Chemokines / biosynthesis*
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Chemokines / chemistry
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Chemokines, CXC*
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Chemotactic Factors / biosynthesis*
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Chemotactic Factors / chemistry
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Chemotactic Factors / pharmacology
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Chemotaxis, Leukocyte
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Culture Media, Conditioned
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Growth Substances / biosynthesis*
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Growth Substances / chemistry
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Growth Substances / pharmacology
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Humans
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Intercellular Signaling Peptides and Proteins*
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Lipopolysaccharides / pharmacology*
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / metabolism*
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Male
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Molecular Sequence Data
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Neutrophils / drug effects
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Neutrophils / physiology
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Rats
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Rats, Wistar
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Sequence Homology, Amino Acid
Substances
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CXCL1 protein, human
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Chemokine CXCL1
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Chemokine CXCL2
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Chemokines
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Chemokines, CXC
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Chemotactic Factors
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Culture Media, Conditioned
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Cxcl1 protein, rat
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Cxcl2 protein, rat
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Cxcl3 protein, rat
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Lipopolysaccharides