Prolactin induces switching of T-cell receptor gene expression from alpha to gamma in rat Nb2 pre-T lymphoma cells(1)

Biochem Biophys Res Commun. 1996 Mar 27;220(3):958-62. doi: 10.1006/bbrc.1996.0514.

Abstract

Prolactin (PRL) is considered to be an immunomodulator. This study was conducted to analyze the actions of PRL on the gene expressions for T-cell receptor (TCR) chains in a fat pre-T lymphoma cell line, Nb2. PRL remarkably stimulated the gene expression for TCR gamma chain within 4 h and suppressed TCR alpha chain mRNA expression within 8 h in Nb2 cells. The expression of TCR beta mRNA in the cells was concomitantly, but to a less extent, repressed by the hormone treatment. These results suggest that PRL may participate in the regulation of mammalian T-cell differentiation and development.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Gene Library
  • Lymphoma, T-Cell
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Prolactin / pharmacology*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Antigen, T-Cell, alpha-beta / biosynthesis*
  • Receptors, Antigen, T-Cell, gamma-delta / biosynthesis*
  • Restriction Mapping
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta
  • Prolactin

Associated data

  • GENBANK/D55648