Abstract
A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Brassica / genetics*
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Cysteine Proteinase Inhibitors / biosynthesis
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Cysteine Proteinase Inhibitors / genetics*
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Cysteine Proteinase Inhibitors / pharmacology
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DNA, Complementary / genetics
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Escherichia coli / genetics
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Gene Dosage
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Genes, Plant
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Molecular Sequence Data
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Multigene Family
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Papain / antagonists & inhibitors
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Plant Proteins / biosynthesis
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Plant Proteins / genetics*
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Plant Proteins / pharmacology
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Plant Shoots
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / pharmacology
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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BCPI-1 protein, Brassica rapa
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Cysteine Proteinase Inhibitors
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DNA, Complementary
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Plant Proteins
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Recombinant Fusion Proteins
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Papain