Gastric lymphoepithelioma-like carcinoma and jejunal B-cell MALT lymphoma with large cell transformation. Demonstration of EBV with identical LMP gene deletions in the carcinoma and large cell lymphoma

Am J Clin Pathol. 1996 May;105(5):560-6. doi: 10.1093/ajcp/105.5.560.

Abstract

The authors describe a patient with gastric lymphoepithelioma-like carcinoma (LELC) and jejunal low grade B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) with histologic transformation to large cell lymphoma. In situ hybridization studies for Epstein-Barr virus (EBV) demonstrated abundant EBV RNA (EBER) within the neoplastic cells of the gastric LELC and the jejunal large cell lymphoma. The low grade MALT lymphoma was EBER negative. Polymerase chain reaction (PCR) studies confirmed the in situ hybridization results, and demonstrated a 30 base pair deletion in the 3' end of the latent membrane protein gene in both the gastric LELC and the jejunal large cell lymphoma. These results suggest that the same viral strain infected both the stomach and jejunal tumors. In addition, the finding of EBV in the large cell lymphoma, but not the low grade component suggests that EBV may have played a role in large cell transformation.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Base Sequence
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology*
  • Carcinoma, Squamous Cell / virology
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / pathology
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Gene Deletion*
  • Herpesvirus 4, Human / genetics*
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Immunohistochemistry
  • Jejunal Neoplasms / genetics
  • Jejunal Neoplasms / pathology*
  • Jejunal Neoplasms / virology
  • Lymphoma, B-Cell, Marginal Zone / genetics
  • Lymphoma, B-Cell, Marginal Zone / pathology*
  • Lymphoma, B-Cell, Marginal Zone / virology
  • Molecular Sequence Data
  • Neoplasms, Multiple Primary / genetics
  • Neoplasms, Multiple Primary / pathology*
  • Neoplasms, Multiple Primary / virology
  • Polymerase Chain Reaction
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / pathology*
  • Stomach Neoplasms / virology
  • Viral Matrix Proteins / genetics*

Substances

  • Viral Matrix Proteins