IL-1Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R AcP

J Biol Chem. 1996 Feb 23;271(8):3967-70. doi: 10.1074/jbc.271.8.3967.

Abstract

A novel member of the interleukin-1 receptor family has been cloned by polymerase chain reaction using degenerate oligonucleotide primers derived from regions of sequence conservation, using as template a yeast artificial chromosome known to contain both interleukin-1 (IL-1) receptors and T1/ST2. The new receptor, called IL-1 receptor-related protein or IL-1Rrp, fails to bind any of the known IL-1 ligands. A chimeric receptor, in which the IL-1Rrp cytoplasmic domain is fused to the extracellular and transmembrane regions of the IL-1 receptor, responds to IL-1 following transfection into COS cells by activation of NFkappaB and induction of IL-8 promoter function.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Membrane / metabolism
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Cytoplasm / metabolism
  • DNA Primers
  • Humans
  • Interleukin-18 Receptor alpha Subunit
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Protein Biosynthesis*
  • Proteins / chemistry*
  • Receptors, Interleukin-1 / biosynthesis*
  • Receptors, Interleukin-1 / chemistry*
  • Receptors, Interleukin-18
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • DNA Primers
  • IL18R1 protein, human
  • Il18r1 protein, mouse
  • Interleukin-18 Receptor alpha Subunit
  • Interleukin-8
  • NF-kappa B
  • Proteins
  • Receptors, Interleukin-1
  • Receptors, Interleukin-18
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/U43672
  • GENBANK/U43673