Abstract
Escherichia coli labile toxin (LT) was assessed as mucosal immunogen and as adjuvant for tetanus toxoid (TT) in mice. After oral administration of LT, C57BL/6 (H-2b) and BALB/c(H-2d) mice were high mucosal and serum antibody responders, while C3H/HeN (H-2k) mice were low responders. High responders exhibited mainly serum IgG (including IgG1, IgG2a, and IgG2b), as well as IgM and IgA, while mucosal responses were IgA. Analysis of LT-B-specific CD4+ T helper (Th) cells from Peyer's patches (PP) or from spleen revealed a mixed Th1 (interferon-gamma) and Th2 (interleukin-4 and -5) cell pattern. Oral LT given with TT induced TT-specific response patterns identical to LT-B. Analysis of mRNA from TT-specific PP CD4+ Th cells also revealed a mixed Th1- and Th2- type response. Thus, antibody response profiles induced by LT are regulated by both CD4+ Th1 and Th2 cell types.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Administration, Oral
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Animals
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Bacterial Toxins / administration & dosage*
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Bacterial Toxins / immunology*
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Cholera Toxin / administration & dosage
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Cholera Toxin / immunology
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Cytokines / genetics
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Cytokines / metabolism
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Enterotoxins / administration & dosage*
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Enterotoxins / immunology*
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Escherichia coli Proteins*
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Female
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Immunization
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Immunoglobulin A / blood
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Immunoglobulin G / blood
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Immunoglobulin G / classification
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Mucous Membrane / immunology
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Peyer's Patches / immunology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Spleen / immunology
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Tetanus Toxoid / administration & dosage
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Tetanus Toxoid / immunology
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Th1 Cells / immunology
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Th2 Cells / immunology
Substances
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Adjuvants, Immunologic
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Bacterial Toxins
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Cytokines
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Enterotoxins
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Escherichia coli Proteins
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Immunoglobulin A
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Immunoglobulin G
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RNA, Messenger
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Tetanus Toxoid
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Cholera Toxin
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heat-labile enterotoxin, E coli