Abstract
Earlier studies showed that purified IgG from sera of rabbits immunized with a boiled Escherichia coli J5 (Rc chemotype) whole cell vaccine protected neutropenic rats against gram-negative bacterial sepsis. In the present study, de-O-acylated J5 lipopolysaccharide (J5 DLPS) as a noncovalent complex with Neisseria meningitidis group B outer membrane protein (GBOMP) elicited anti-J5 LPS antibodies in rabbits. IgG prepared from immune rabbit sera protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Sixteen of 26 rats treated with the postimmune serum IgG were protected compared with none of 20 rats treated with the control rabbit serum IgG (P < .001). In vitro binding studies showed binding of anti-J5 IgG to several gram-negative bacteria. These results indicate that a subunit vaccine made of J5 DLPS as a noncovalent complex with GBOMP may protect against gram-negative bacteremia.
MeSH terms
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Animals
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Antibodies, Bacterial / blood
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Antibodies, Bacterial / immunology
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Antibody Specificity
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Bacteremia / immunology
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Bacteremia / prevention & control*
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Vaccines / immunology*
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Endotoxins / blood
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Escherichia coli / immunology*
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Escherichia coli Infections / immunology
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Escherichia coli Vaccines
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Female
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Gram-Negative Bacterial Infections / immunology
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Gram-Negative Bacterial Infections / prevention & control*
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Immune Sera / immunology
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Immunization, Passive
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Immunoglobulin G / blood
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Immunoglobulin G / immunology
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Lipopolysaccharides / chemistry
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Lipopolysaccharides / immunology*
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Neisseria meningitidis / immunology*
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Pseudomonas Infections / prevention & control
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Rabbits
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Rats
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Rats, Sprague-Dawley
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Vaccines, Conjugate / immunology
Substances
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Antibodies, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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Endotoxins
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Escherichia coli Vaccines
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Immune Sera
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Immunoglobulin G
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Lipopolysaccharides
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Vaccines, Conjugate