Abstract
Mast cells originate from hematopoietic stem cells, but the mast cell-committed precursor has not been identified. In the study presented here, a cell population in murine fetal blood that fulfills the criteria of progenitor mastocytes was identified. It is defined by the phenotype Thy-1loc-Kithi, contains cytoplasmic granules, and expresses RNAs encoding mast cell-associated proteases but lacks expression of the high-affinity immunoglobulin E receptor. Thy-1loc-Kithi cells generated functionally competent mast cells at high frequencies in vitro but lacked developmental potential for other hematopoietic lineages. When transferred intraperitoneally, this population reconstituted the peritoneal mast cell compartment of genetically mast cell-deficient W/Wv mice to wild-type levels.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Lineage
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Cell Transplantation
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Cells, Cultured
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Cytoplasmic Granules / ultrastructure
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Endopeptidases / genetics
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Endopeptidases / metabolism
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Fetal Blood
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / physiology
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Hematopoietic Stem Cells / ultrastructure
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Immunophenotyping
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Interleukin-3 / pharmacology
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Mast Cells / cytology*
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Mast Cells / physiology
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Mast Cells / ultrastructure
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Mice
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Molecular Sequence Data
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Peritoneal Cavity / cytology
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Proto-Oncogene Proteins c-kit / analysis
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, IgE / analysis
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Receptors, IgE / genetics
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Stem Cell Factor / pharmacology
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Thy-1 Antigens / analysis
Substances
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Interleukin-3
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RNA, Messenger
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Receptors, IgE
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Stem Cell Factor
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Thy-1 Antigens
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Proto-Oncogene Proteins c-kit
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Endopeptidases