Use of immobilized synthetic peptides for the identification of contact sites between human interleukin-6 and its receptor

FEBS Lett. 1996 Jan 29;379(2):122-6. doi: 10.1016/0014-5793(95)01482-9.

Abstract

Synthetic peptides immobilized on cellulose membranes proved to be a powerful tool for the identification of sites in the cytokine IL-6 involved in receptor binding. Similarly, a region in the extracellular part of the IL-6 receptor which is important for interaction with its ligand was identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / chemistry*
  • Antigens, CD / isolation & purification
  • Antigens, CD / metabolism*
  • Binding Sites
  • Cloning, Molecular
  • Escherichia coli
  • Humans
  • Interleukin-6 / chemistry*
  • Interleukin-6 / isolation & purification
  • Interleukin-6 / metabolism*
  • Models, Structural
  • Molecular Sequence Data
  • Peptides / chemistry
  • Protein Structure, Secondary*
  • Receptors, Interleukin / chemistry*
  • Receptors, Interleukin / isolation & purification
  • Receptors, Interleukin / metabolism*
  • Receptors, Interleukin-6
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism

Substances

  • Antigens, CD
  • Interleukin-6
  • Peptides
  • Receptors, Interleukin
  • Receptors, Interleukin-6
  • Recombinant Proteins