Abstract
A DNA probe containing the structural gene for dicarboxylate transport (dct A) of Rhizobium meliloti hybridized strongly with the fragments of Azospirillum lipoferum genomic DNA. A genomic library of A. lipoferum was screened for the dct A gene by complementation of a dct A mutant of Rhizobium meliloti. A recombinant cosmid, p37D, capable of restoring growth of the dct A mutant on dicarboxylates was isolated and found to hybridize to the dctA probe. The ability of p37D to complement the dct B mutant of R. meloliti indicated that dct A and dct B genes in A. lipoferum may be organized adjacent to each other.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azospirillum / genetics*
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Bacterial Proteins*
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Biological Transport, Active / genetics
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cloning, Molecular
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Cosmids
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DNA Probes
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DNA, Bacterial / genetics
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Dicarboxylic Acid Transporters*
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Dicarboxylic Acids / metabolism
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Genes, Bacterial
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Genetic Complementation Test
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Multigene Family
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Mutation
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Restriction Mapping
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Sinorhizobium meliloti / genetics*
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Sinorhizobium meliloti / metabolism
Substances
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Bacterial Proteins
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Carrier Proteins
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DNA Probes
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DNA, Bacterial
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Dicarboxylic Acid Transporters
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Dicarboxylic Acids
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Receptors, Cell Surface
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dctA protein, Sinorhizobium meliloti
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DCTB protein, Sinorhizobium
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Protein Kinases