+P5 (D1S3309E), a novel target binding site for the Wilms' tumour suppressor 1 (WT1) gene, maps to human chromosome 1q21-->22

K Negus; G H Holmes; C Wicking; B J Wainwright; M H Little

doi:10.1159/000134210

+P5 (D1S3309E), a novel target binding site for the Wilms' tumour suppressor 1 (WT1) gene, maps to human chromosome 1q21-->22

Cytogenet Cell Genet. 1996;72(4):306-9. doi: 10.1159/000134210.

Authors

K Negus¹, G H Holmes, C Wicking, B J Wainwright, M H Little

Affiliation

¹ Centre for Molecular and Cellular Biology, University of Queensland, St. Lucia, Australia.

PMID: 8641136
DOI: 10.1159/000134210

Abstract

The Wilms' tumor suppressor 1 gene (WT1) encodes a zinc finger transcription factor critical for normal urogenital development. We have previously isolated a DNA fragment, +P5 (D1S3309E), to which all WT1 protein isoforms bind. Using PCR of a human x rodent somatic cell hybrid mapping panel, together with two-color fluorescence in situ hybridisation of +P5-containing cosmids and previously localised human chromosome 1q cosmids, we have mapped the +P5 fragment to chromosome 1q21-->q22.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Binding Sites / genetics
Chromosome Mapping
Chromosomes, Human, Pair 1 / genetics*
Chromosomes, Human, Pair 1 / ultrastructure
Cosmids
DNA / genetics*
DNA / metabolism*
DNA Primers / genetics
DNA-Binding Proteins / genetics*
Genes, Wilms Tumor*
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
Transcription Factors / genetics*
WT1 Proteins
Zinc Fingers / genetics*

Substances

DNA Primers
DNA-Binding Proteins
Transcription Factors
WT1 Proteins
DNA

Associated data

GENBANK/U60334