Abstract
In previous experiments we have shown the role of nitric oxide (NO) in basal and interleukin-1 beta (IL-1 beta)-induced CRH and ACTH release in vitro. Now, we have studied the possible production of NO from hypothalamic cell cultures, particularly after IL-1 beta stimulation or L-NOArg inhibition, by high performance liquid chromatographic (HPLC) assay of L-citrulline production, adding further evidence for a role of NO in IL-1 beta activity in the hypothalamus.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Arginine / analogs & derivatives
-
Arginine / pharmacology
-
Cells, Cultured
-
Chromatography, High Pressure Liquid
-
Citrulline / biosynthesis
-
Enzyme Inhibitors / pharmacology
-
Hypothalamus / cytology
-
Hypothalamus / metabolism*
-
Interleukin-1 / physiology*
-
Nitric Oxide / biosynthesis*
-
Nitric Oxide Synthase / antagonists & inhibitors
-
Nitroarginine
-
Rats
Substances
-
Enzyme Inhibitors
-
Interleukin-1
-
Nitroarginine
-
Citrulline
-
Nitric Oxide
-
Arginine
-
Nitric Oxide Synthase