Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay

Am J Hum Genet. 1996 Jun;58(6):1239-46.

Abstract

The use of DNA typing in human genome analysis is increasing and finding widespread application in the area of forensic and paternity testing. In this report, we explore the feasibility of typing single nucleotide polymorphisms (SNPs) by using a semiautomated method for analyzing human DNA samples. In this approach, PCR is used to amplify segments of human DNA containing a common SNP. Allelic nucleotides in the amplified product are then typed by a colorimetric implementation of the oligonucleotide ligation assay (OLA). The results of the combined assay, PCR/OLA, are read directly by a spectrophotometer; the absorbances are compiled; and the genotypes are automatically determined. A panel of 20 markers has been developed for DNA typing and has been tested using a sample panel from the CEPH pedigrees (CEPH parents). The results of this typing, as well as the potential to apply this method to larger populations, are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human
  • Colorimetry / methods
  • DNA / analysis
  • DNA Primers
  • DNA Probes
  • Feasibility Studies
  • Female
  • Forensic Medicine / methods*
  • Genetic Markers
  • Genetic Variation
  • Genome, Human*
  • Genotype
  • Humans
  • Male
  • Molecular Sequence Data
  • Paternity*
  • Pedigree
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Primers
  • DNA Probes
  • Genetic Markers
  • DNA