Posterior patterning by the Caenorhabditis elegans even-skipped homolog vab-7

Genes Dev. 1996 May 1;10(9):1120-30. doi: 10.1101/gad.10.9.1120.

Abstract

Patterning of the posterior end in animals is not well understood. Homologs of Drosophila even-skipped (eve) have a similar posterior expression pattern in many animals, and in vertebrates they are linked physically to the "posterior" ends of homeotic clusters (HOM-C), suggesting a conserved role in posterior development. However, the function of this posterior expression is not known. Here I show that the Caenorhabditis elegans gene vab-7 encodes an eve homolog that is required for posterior development and expressed in a pattern strikingly similar to that of vertebrate eve genes. Using a four-dimensional recording system, I found that posterior body muscles and the posterior epidermis are patterned abnormally in vab-7 mutants, but commitment to muscle and epidermal fates is normal. Furthermore, vab-7 activity is required for the complete expression of the most posterior HOM-C gene egl-5 in muscle cells, supporting the idea that eve homologs may act with the HOM-C to determine posterior cell fates. The conservation of sequence and expression pattern between vab-7 and eve homologs in other animals argues that most eve genes have posterior mesodermal and ectodermal patterning functions.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins*
  • Base Sequence
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans Proteins*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Drosophila / genetics
  • Drosophila Proteins*
  • Epidermis / embryology
  • Gene Expression Regulation, Developmental*
  • Genes, Helminth / physiology
  • Helminth Proteins / genetics
  • Homeodomain Proteins / chemistry
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / physiology
  • Microscopy, Video
  • Molecular Sequence Data
  • Muscles / embryology
  • Mutation
  • RNA, Helminth / biosynthesis
  • RNA, Messenger / biosynthesis
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • Bacterial Proteins
  • Caenorhabditis elegans Proteins
  • DNA, Complementary
  • Drosophila Proteins
  • Helminth Proteins
  • Homeodomain Proteins
  • RNA, Helminth
  • RNA, Messenger
  • Transcription Factors
  • VAB-7 protein, C elegans
  • eve protein, Drosophila

Associated data

  • GENBANK/X96636