Abstract
A yeast artificial chromosome (YAC) contig has been constructed in 16p12.1-p11.2 that encompasses three loci (D16S288, D16S299, and D16S298) closely linked to the gene causing Batten disease or juvenile-onset neuronal ceroid lipofuscinosis (CLN3). The physical map has been ordered using 42 sequence tagged sites. Four genes, interleukin-4 receptor (IL4R), phenol-preferring phenol sulfotransferase (STP), monoamine-preferring phenol sulfotransferase (STM), and sialophorin (SPN), have been mapped to the YAC contig. A partial genomic restriction map has been constructed to confirm the order and distances between D16S298, predicted to be the locus closest to CLN3. The overlapping genomic clones are a valuable resource for cloning the Batten gene (CLN3) and other genes in the region.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / genetics
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Arylsulfotransferase / genetics
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Base Sequence
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Chromosome Mapping
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Chromosomes, Artificial, Yeast
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Chromosomes, Human, Pair 16*
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Cosmids
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DNA Primers
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Genetic Linkage
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Genetic Markers
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Humans
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In Situ Hybridization, Fluorescence
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Interleukin-4 / metabolism
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Isoenzymes / genetics
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Leukosialin
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Molecular Sequence Data
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Neuronal Ceroid-Lipofuscinoses / genetics*
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Polymerase Chain Reaction / methods
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Receptors, Interleukin / genetics
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Receptors, Interleukin-4
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Restriction Mapping
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Sequence Tagged Sites
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Sialoglycoproteins / genetics
Substances
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Antigens, CD
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DNA Primers
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Genetic Markers
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Isoenzymes
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Leukosialin
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Receptors, Interleukin
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Receptors, Interleukin-4
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SPN protein, human
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Sialoglycoproteins
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Interleukin-4
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Arylsulfotransferase