Abstract
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its amino-terminus and a putative membrane-spanning region at its carboxy-terminus.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Ascorbate Peroxidases
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Base Sequence
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Chloroplasts / enzymology
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics*
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DNA, Plant / genetics*
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Fruit / enzymology*
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Fruit / genetics*
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Molecular Sequence Data
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Peroxidases / genetics*
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Peroxidases / metabolism
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Sequence Homology, Amino Acid
Substances
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DNA Primers
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DNA, Complementary
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DNA, Plant
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Peroxidases
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Ascorbate Peroxidases