Allelic dropout caused by allele-specific amplification failure in single-cell PCR of the cystic fibrosis delta F508 deletion

MeSH terms

• Alleles*
• Artifacts*
• Cystic Fibrosis / diagnosis*
• Cystic Fibrosis / genetics
• Cystic Fibrosis / prevention & control
• Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
• DNA / genetics*
• DNA / isolation & purification
• DNA Mutational Analysis / methods*
• Feasibility Studies
• Heterozygote
• Homozygote
• Humans
• Leukocytes / chemistry*
• Polymerase Chain Reaction / methods*
• Prenatal Diagnosis / methods*
• Sequence Deletion*