Expression of Na+/myo-inositol cotransporter mRNA in normal and hypertonic stress rat eyes

Brain Res Mol Brain Res. 1996 Jan;35(1-2):333-8. doi: 10.1016/0169-328x(95)00245-n.

Abstract

We studied the localization of Na+/myo-inositol cotransporter (SMIT) mRNA in normal and hypertonic stress rat eyes by in situ hybridization histochemistry using cRNA probes. SMIT mRNA signals were observed in the iris-ciliary body, the lens epithelial cells, and the ganglion cell layer and the inner nuclear layer of the retina. There was a rapid increase on SMIT mRNA in the retina of hypertonic stress rats compared with control rats. These findings suggest that Na+/myo-inositol cotransporter gene expression is osmotically regulated in vivo to protect retinal neuronal function against hypertonic stress.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Carrier Proteins / biosynthesis*
  • Ciliary Body / metabolism
  • Eye / metabolism*
  • Gene Expression*
  • Heat-Shock Proteins / biosynthesis*
  • In Situ Hybridization
  • Inositol / metabolism
  • Lens, Crystalline / metabolism
  • Male
  • Membrane Proteins*
  • RNA Probes
  • RNA, Complementary
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Wistar
  • Reference Values
  • Retina / metabolism
  • Retinal Ganglion Cells / metabolism
  • Saline Solution, Hypertonic*
  • Sodium / metabolism
  • Stress, Physiological*
  • Symporters*

Substances

  • Carrier Proteins
  • Heat-Shock Proteins
  • Membrane Proteins
  • RNA Probes
  • RNA, Complementary
  • RNA, Messenger
  • Saline Solution, Hypertonic
  • Symporters
  • SLC5A3 protein, human
  • Inositol
  • Sodium