Abstract
We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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CCAAT-Enhancer-Binding Proteins
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Cats
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Cell Line
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation, Viral*
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Immediate-Early Proteins / biosynthesis
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Immediate-Early Proteins / metabolism*
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Immunodeficiency Virus, Feline / genetics*
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Immunodeficiency Virus, Feline / metabolism
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Kidney
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Nuclear Proteins / metabolism*
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Plasmids
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Repetitive Sequences, Nucleic Acid*
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Simplexvirus*
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Transcription Factors
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Transfection
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Immediate-Early Proteins
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Nuclear Proteins
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Recombinant Proteins
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Transcription Factors
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herpes simplex virus, type 1 protein ICP4