Abstract
A 5-h PCR assay (Amplicor enterovirus test) was compared with viral culture for the detection of enteroviruses in cerebrospinal fluid. Of the cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 34% were positive by viral culture whereas 66% were positive by the Amplicor PCR, suggesting that this technique improves the diagnosis of enteroviral meningitis.
MeSH terms
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Base Sequence
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Cerebrospinal Fluid / virology
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Child
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DNA Primers / genetics
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Disease Outbreaks
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Enterovirus / genetics*
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Enterovirus / isolation & purification*
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Enterovirus Infections / diagnosis
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Enterovirus Infections / epidemiology
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Enterovirus Infections / virology
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Evaluation Studies as Topic
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Humans
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Meningitis, Aseptic / epidemiology
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Meningitis, Aseptic / virology*
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Meningitis, Viral / diagnosis
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Meningitis, Viral / epidemiology
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Meningitis, Viral / virology
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Prospective Studies
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RNA, Viral / cerebrospinal fluid
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RNA, Viral / genetics
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Virology / methods*
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Virus Cultivation